5 Common methods for gonorrhea test

5 Common methods for gonorrhea test
1.Smear: Patients with urethral discharge, as gram staining, gram negative diplococcus found in polymorphonuclear cells. There are a large number of smears of purulent secretions of simple gonorrhea patients, the positive rate was about 90%; the secretions of less chronic gonorrhea patients, the positive rate is low, so to get prostate massage fluid, to improve detection the rate of.

2.Training examination: Neisseria gonorrhoeae culture is an important evidence of diagnosis, the method of cultivation symptoms are mild or asymptomatic male and female patients are more sensitive, as long as the culture positive can be diagnosed. Before the advent of gene diagnosis, culture is the only method for the screening of gonorrhea recommended by WHO.

3.Antigen detection: includes two kinds, one is the solid phase enzyme immunoassay, can be used for the detection of Neisseria gonorrhoeae antigen in clinical specimens; two is the direct immunofluorescence test by monoclonal antibody detection of Neisseria gonorrhoeae I direct immunofluorescence test. The former can be used in the diagnosis of gonococcal infection in the population of women, the latter is not experimental recommended.

4.Drug sensitivity test: in the culture of positive backward one step for drug sensitivity test. Using disk diffusion method to do sensitive tests, or agar plate dilution method to determine the minimum inhibitory concentration (MIC), used to guide the use of antibiotics.


5.PPNG detection: β-lactamase,using paper acidity quantitative method, using I PP-NG filter paper Whatman strain can make its color from blue to yellow, positive for PPNG, negative for N-PPNG.

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